Recombinant Human Pancreasin / Marapsin / PRSS27 Protein (His tag)

• Catalog number
  PKSH030900-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  CAPH2;MPN;UNQ1884/PRO4327
• Activity
  Measured by its ability to cleave a colorimetric peptide substrate, NcarbobenzyloxyGlyArgThioBenzyl ester (ZGR-SBzl), in the presence of 5,5’Dithiobis (2-nitrobenzoic acid) (DTNB).The specific activity is > 2000 pmols/min/μg.
• Sequence
  Met 1-Lys 290
• Fusion tag
  C-His
• Accession
  Q9BQR3
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  30.9 kDa
• AP Mol Mass
  42 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), which is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Pancreasin   Marapsin   PRSS27   Protein   His   tag  
• Gene symbol
  PRSS27
• Short name
  Recombinant Pancreasin / Marapsin / PRSS27 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Pancreasin / Marapsin / PRSS27 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:15475
• Gene
  PRSS27
• Long gene name
  serine protease 27
• Synonyms gene name
  • protease, serine 27
• Synonyms
  • MPN
  • CAPH2
• Synonyms name
  • pancreasin
  • marapsin
• GenBank acession
  • AB056161
• Locus
  16p13.3
• Discovery year
  2001-05-10
• Entrez gene record
  83886
• RefSeq identity
  • NM_031948
• Classification
  • Serine proteases
• VEGA ID
  OTTHUMG00000128929

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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