Recombinant Human Stem Cell Factor/SCF/c-Kit Ligand (C-6His)

• Catalog number
  CX53
• Price
  Please ask
• Size
  1mg

• Alternative names
  Kit Ligand, Mast Cell Growth Factor, MGF, Stem Cell Factor, SCF, c-Kit ligand, KITLG, MGF, SCF
• Description
  Recombinant Human Stem Cell Factor is produced by our Mammalian expression system and the target gene encoding Glu26-His214 is expressed with a 6His tag at the C-terminus.
• Expression
  Human Cells
• Tag
  C-His
• Endotoxin
  Less than 0.1 ng/ug (1 EU/ug)
• Accession
  P21583
• TargetSequence
  Glu26-His214
• Shipping
  Ambient
• Formulation
  Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
• Note
  For research use only.
• Additional description
  For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants
• Test
  Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.

• Gene target
  Stem   Factor/SCF/c-Kit   Ligand   C-6His  
• Gene symbol
  KITLG
• Short name
  Recombinant Stem Factor/SCF/c-Kit Ligand (C-6His)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens progenitor cellular Factor/SCF/c-reagent Ligand (C-6His)
• Alternative technique
  rec, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell, stem

Gene info

• Identity
  HGNC:6343
• Gene
  KITLG
• Long gene name
  KIT ligand
• Synonyms gene
  • MGF
• Synonyms
  • SCF
  • SF
  • Kitl
  • KL-1
  • FPH2
  • SLF
  • DFNA69
• Synonyms name
  • mast cell growth factor
  • stem cell factor
  • steel factor
  • familial progressive hyperpigmentation 2
• GenBank acession
  • M59964
• Locus
  12q21.32
• Discovery year
  1991-06-04
• Entrez gene record
  4254
• Pubmed identfication
  • 2208279
  • 1707188
  • 19375057
  • 26522471
• RefSeq identity
  • NM_003994
• VEGA ID
  OTTHUMG00000169888
• Locus Specific Databases
  • LRG_1379

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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