Recombinant Human Endothelial Cell Adhesion Molecule/ESAM (C-Fc)

Alternative names

Endothelial Cell-Selective Adhesion Molecule, ESAM
Description

Recombinant Human ESAM is produced by our Mammalian expression system and the target gene encoding Gln30-Ala247 is expressed with a Fc tag at the C-terminus.
Expression

Human Cells
Tag

C-Fc
Endotoxin

Less than 0.1 ng/ug (1 EU/ug)
Accession

Q96AP7
TargetSequence

Gln30-Ala247
Shipping

Ambient
Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Note

For research use only.
Additional description

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Recombinant Endothelial Adhesion Molecule/ESAM (C-Fc). For cells, cell lines and tissues in culture till half confluency. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Endothelial Adhesion Molecule/ESAM C-Fc
Gene symbol

ESAM, ESAM-AS1
Short name

Recombinant Endothelial Adhesion Molecule/ESAM (C-Fc)
Technique

Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Species

Human, Humans
Alternative name

Rec. H. sapiens Endothelial cellular Adhesion Molecule/endothelial cell adhesion molecule (C-fragment c)
Alternative technique

rec
Alternative to gene target

endothelial cell adhesion molecule, ESAM and IDBG-75276 and ENSG00000149564 and 90952, protein binding, Cell surfaces, Esam and IDBG-150411 and ENSMUSG00000001946 and 69524, BT.54007 and IDBG-642621 and ENSBTAG00000001004 and 616926
Tissue

cell

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data