Recombinant Human Cytotoxic T-lymphocyte Protein 4(C-Flag)

• Catalog number
  CS47
• Price
  Please ask
• Size
  1mg

• Alternative names
  Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4
• Description
  Recombinant Human Cytotoxic T-lymphocyte Protein 4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a Flag tag at the C-terminus.
• Expression
  Human cells
• Tag
  C-Flag
• Endotoxin
  Less than 0.1 ng/ug (1 EU/ug)
• Accession
  P16410
• TargetSequence
  Lys36-Asp161
• Shipping
  Ambient
• Formulation
  Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
• Note
  For research use only.
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Cytotoxic   T-lymphocyte   Protein  
• Short name
  Recombinant Cytotoxic T-lymphocyte Protein 4(C- )
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Bon Opus advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  Flag
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Cytotoxic T-lymphocyte Protein 4(C-Flag)
• Alternative technique
  rec
• Tissue
  lymphocyte

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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