Smad3 (phospho-S213) polyclonal antibody
-
Catalog number
AP0329-50ul
-
Price
Please ask
-
Size
50ul
-
-
Alternative Name
Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
-
Immunogen
Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 213.
-
Swiss Prot
P84022
-
Reactivity
Human,Mouse,Rat
-
Applications
WB
-
Application_all
WB: 1:500~1:1000
-
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
-
Purification Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
-
Storage Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
-
Specificity
p-Smad3 (S213) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser213.
-
Usage
Western blot (WB) analysis of p-Smad3 (S213) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(20ug) Lane4:LOVO whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug) Lane6:AML-12 whole cell lysate(40ug)
-
BiowMW
~ 48, 55 kDa
-
Note
For research use only, not for use in diagnostic procedure.
-
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Short name
Smad3 (phospho-S213) polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Host
Rabbit
-
Alternative name
SMAD family member 3 (phosphorilated-S213) polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products