RUNX1/2/3 polyclonal antibody

Alternative Name

CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B, RUNX1, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2, CBF-alpha-3, PEA2-alpha C, PEBP2-alpha C, RUNX3,
Immunogen

recombinant protein
Swiss Prot

Q01196(Human) Q13761(Human) Q13950(Human) Q03347(Mouse) Q08775(Mouse) Q64131(Mouse) Q63046(Rat) Q9Z2J9(Rat)
Reactivity

Human, Mouse, Rat
Applications

WB ICC/IF IHC IP FC
Application_all

WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100
Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification Purity

ProA affinity purified
Storage Stability

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity

RUNX1/2/3 polyclonal antibody detects endogenous levels of RUNX1/2/3 protein.
Usage

Western blot analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.
BiowMW

49 kDa
Note

For research use only, not for use in diagnostic procedure.
Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

RUNX1/2/3
Gene symbol

RUNX1-IT1, RUNX1
Short name

RUNX1/2/3 polyclonal antibody
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Host

Rabbit
Alternative name

runt-related transcription factor 1/2/3 polyclonal (antibody to-)
Alternative technique

polyclonals, antibodies
Alternative to gene target

runt-related transcription factor 1, AML1 and AML1-EVI-1 and AMLCR1 and CBFA2 and EVI-1 and PEBP2aB, RUNX1 and IDBG-2825 and ENSG00000159216 and 100506403,101928269,861, repressing transcription factor binding, nuclei, Runx1 and IDBG-177713 and ENSMUSG00000022952 and 12394, RUNX1 and IDBG-640443 and ENSBTAG00000004742 and 529631

Identity

HGNC:16623
Gene

RUNX1-IT1
Long gene name

RUNX1 intronic transcript 1
Synonyms gene
- C21orf96
Synonyms gene name
- chromosome 21 open reading frame 96
- RUNX1 intronic transcript 1 (non-protein coding)
Synonyms
- FLJ20856
GenBank acession
- AK024509
Locus

21q22.12
Discovery year

2003-10-01
Entrez gene record

80215
Pubmed identfication
- 32487998
RefSeq identity
- NR_026812
Classification
- Intronic transcripts

Identity

HGNC:10471
Gene

RUNX1
Long gene name

RUNX family transcription factor 1
Synonyms gene
- AML1
- CBFA2
Synonyms gene name
- acute myeloid leukemia 1
- runt-related transcription factor 1
- runt related transcription factor 1
Synonyms
- PEBP2A2
- AMLCR1
Synonyms name
- aml1 oncogene
GenBank acession
- X79549
Locus

21q22.12
Discovery year

1991-08-20
Entrez gene record

861
Pubmed identfication
- 1427868
- 7835892
Classification
- Runt-related transcription factors
VEGA ID

OTTHUMG00000086299
Locus Specific Databases
- LRG_482

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data