WST-NR-CV Combined Cytotoxicity Assay Kit

• Catalog number
  K543-1000
• Price
  Please ask
• Size
  1000 assays

• Description
  Sensitive, Non-radioactive Assay, measures cytotoxicity using 3 different methods
• Summary
  • Detection- Absorbance [OD: WST/NR/CV: 450 /540/570 nm • Species reactivity- Mammalian • Kit size- 1000 • Applications- The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
• Detection Method
  Absorbance [OD: WST/NR/CV: 450 /540/570 nm
• Species Reactivity
  Eukaryotes
• Applications
  The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
• Sample Type
  Cell cultures (adherent and suspension)
• Features Benefits
  • Simple procedures; take less than 4 hours • Convenient • Uses a water-soluble tetrazolium salt WST reagent, Neutral Red and Crystal Violet . Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. BioVision’s WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  WST-NR-CV   Combined   Cytotoxicity   Kit  
• Short name
  WST-NR-CV Combined Cytotoxicity Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  WST-NR-CV Combined Cytotoxicity test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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