Bioluminescence Cytotoxicity Assay Kit

Description

Cytotoxicity assay based on adenylate kinase (AK), HTS
Summary

• Detection method- Luminescence • Species reactivity- Mammalian
Sample Type

Cell cultures (adherent and suspension)
Features Benefits

• Simple one-step procedure; takes less than 30 minutes • Fast and convenient • The assay is highly sensitive and can be fully automatic for high throughput studies.
Storage Conditions

-20°C
Shipping Conditions

gel pack
Shelf life

12 months
Background

Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and rapidly release into culture medium upon damage of the plasma membrane. The Bioluminescence Cytotoxicity Assay Kit is based on the measurement of AK in a simple one-step procedure involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase that was released from the damaged cells. The second reaction utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is highly sensitive and can be fully automatic for high throughput.
Data Sheet Link
Test

Biovision supplies other types of Assays as 1.

Gene target

Bioluminescence Cytotoxicity Kit
Short name

Bioluminescence Cytotoxicity Assay Kit
Technique

Assay, assays
Host

Assay
Alternative name

Bioluminescence Cytotoxicity test reagent
Alternative technique

arrays, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832