Tyrosinase Activity Assay Kit (Colorimetric)

• Catalog number
  K742-100
• Price
  Please ask
• Size
  100 assays

• Description
  The first non-radioactive kit on the market that measure Tyrosinase activity in biological samples
• Summary
  • Detection method- Absorbance (510 nm)• Applications- Measurement of Tyrosinase Activity in various tissue/cell lysates- Analysis of Tyrosinase activity in food chemistry/skin-related research
• Detection Method
  Absorbance (510 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Measurement of Tyrosinase Activity in various tissue/cell lysates• Analysis of Tyrosinase activity in food chemistry/skin-related research
• Sample Type
  • Animal tissues: liver, heart, kidney, etc.• Cell culture: Adherent and suspension cells (e.g melanoma cells)• Plant tissues: potato, etc.• Purified/recombinant protein/preparations
• Features Benefits
  • First non-radioactive assay in the market• Simple, rapid & convenient• The assay can detect Tyrosinase Activity less than 30 µU in biological samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments. BioVision’s Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 µU Tyrosinase in biological samples
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Tyrosinase   Activity   Kit  
• Short name
  Tyrosinase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Tyrosinase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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