Fumarase Activity Colorimetric Assay Kit

• Catalog number
  K596-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only kit on the market to measure Fumarase Activity
• Summary
  • Detection method- Absorbance (OD 450 nm) • Applications- - Measurement of fumarase activity in various tissues/cells. - Analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.
• Detection Method
  Absorbance (OD 450 nm)
• Applications
  • Measurement of fumarase activity in various tissues/cells. • Analysis of citric acid cycle, reductive carboxylation cycle (CO2 fixation) and renal cell carcinomas.
• Sample Type
  • Animal tissues: liver, heart, muscle etc. • Isolated mitochondria. • Cell culture: Adherent or suspension cells.
• Features Benefits
  • Simple & Rapid Protocol • High-Throughput • This kit can detect less than 50 U/ml of fumarase activity in variety of sample types
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Fumarase (Fumarate Hydratase: EC 4.2.1.2) is an enzyme that catalyzes the reversible reaction of hydration and dehydration of fumarate to Malate. It has two forms: mitochondrial and cytosolic forms. The mitochondrial form of fumarase is one of the key enzymes in citric acid cycle, while cytosolic form is important for metabolism of amino acids and fumarate. In humans, fumarase deficiency leads to serious health problems such as fetal brain abnormality, hypotonia, and renal cell carcinoma. Therefore, accurate measurement of fumarase activity is important for preventing, diagnosis and mechanistic study of fumarase deficiency. Biovision’s Fumarase Activity Assay kit provides a quick and easy method for monitoring fumarase activity in various samples. In this kit, fumarase converts fumarate into malate, which then reacts with Enzyme Mix to form an intermediate. The intermediate subsequently reduces the Developer to a colored product with strong absorbance at 450 nm. The assay is simple and high-throughput ready and can detect less than 50 U/ml of fumarase activity in variety of sample types.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Fumarase   Activity   Kit  
• Short name
  Fumarase Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Fumarase Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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