Sushi Antibody
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Catalog number5010-100
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PricePlease ask
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Size100 µg
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Antibody TargetSushi 3
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Target Alternative NameFactor C Sushi 3 antibody, Sushi 3 antibody
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Antibody TypePolyclonal
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Species ReactivitiesAll
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Immunogen SequenceSushi domain in from E. coli
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) polyclonal antibody in PBS pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern Blot
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Positive ControlRecombinant Sushi protein
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Application And UsagesWestern Blot analysis (1-4 µg/ml).
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DescriptionSushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSRPX, SUSD4, SUSD5
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Short nameSushi Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameSushi (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namesushi repeat containing protein X-linked
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Synonyms gene name
- sushi-repeat-containing protein, X chromosome
- sushi-repeat-containing protein, X-linked
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-09-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namesushi domain containing 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-04-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sushi domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesushi domain containing 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-13
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Entrez gene record
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RefSeq identity
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Classification
- Sushi domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data