Streptavidin - FITC
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Catalog numberP1498-50
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PricePlease ask
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Size50 mg
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DescriptionA non-glycosylated protein which is widely used to bridge biotynlated probes and enzymes.
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RecombinantFalse
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SourceE. coli
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Purity by SDS PAGE>95%
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AssaySDS-PAGE
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Binding Capacity≥ 12 biotin/mg Streptavidin Note: The binding capacity/ activity is determined using BioVision’s Biotin Quantitation Kit (Colorimetric) Cat# K811 in 0.2 M Phosphate buffer pH 7.0, based on biotin binding (HABA) assay. The theoretical binding activity is 14 biotin/mg of streptavidin.
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Molecular Weight~56 KDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized
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Reconstitution Instructions≥ 10 mg/ml in water or PBS.
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Background InformationStreptavidin is a non-glycosylated protein originally isolated from bacterium Streptomyces avidinii. With a very high affinity for biotin, it is widely used to bridge biotinylated probes and enzymes. FITC labeled streptavidin is widely applied in flow cytometry, immunofluorescence cell staining, fluorescence in situ hybridization and other biological assays.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesThis Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Gene target
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Short nameStreptavidin - FITC
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TechniqueFITC, Fluorescein
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Labelstreptavidin
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Alternative namebiotinilated-binding streptavidin - fluorecein
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data