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Description
A non-glycosylated protein which is widely used to bridge biotynlated probes and enzymes.
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Recombinant
False
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Source
E. coli
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Purity by SDS PAGE
>95%
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Assay
SDS-PAGE
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Binding Capacity
≥ 12 biotin/mg Streptavidin Note: The binding capacity/ activity is determined using BioVision’s Biotin Quantitation Kit (Colorimetric) Cat# K811 in 0.2 M Phosphate buffer pH 7.0, based on biotin binding (HABA) assay. The theoretical binding activity is 14 biotin/mg of streptavidin.
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Molecular Weight
~56 KDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized
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Reconstitution Instructions
≥ 10 mg/ml in water or PBS.
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Background Information
Streptavidin is a non-glycosylated protein originally isolated from bacterium Streptomyces avidinii. With a very high affinity for biotin, it is widely used to bridge biotinylated probes and enzymes. FITC labeled streptavidin is widely applied in flow cytometry, immunofluorescence cell staining, fluorescence in situ hybridization and other biological assays.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
