Proteinase K, Recombinant, Molecular Grade
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Catalog number
9210-500
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Price
Please ask
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Size
500 mg
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Synonyms
Endopeptidase K, Tritirachium alkaline proteinase
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Alternative_names
Protease K, Endopeptidase K, Tritirachium alkaline proteinase, Molecular Biology grade Proteinase K
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Description
A serine protease which inactivates nucleases
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Recombinant
Yes
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Source
Tritirachium album
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Activity Specifications test method
>30 units/mg
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Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at 37°C, pH 7.5 (color by Folin-Ciocalteu reagent).
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Molecular Weight
28.9 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized with no additives
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Reconstitution Instructions
In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0.
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Background Information
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Proteinase K, Recombinant, Molecular Grade
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
Proteinase K, Rec., Molecular Grade
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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