Protein G Magnetic Beads

Description

Reusable Protein G conjugated magnetic beads with ≥15 mg/ml binding capacity for antibody purification from multiple sources. Protein G Magnetic Beads are prepared by covalently coupling Recombinant Protein G (contains three IgG binding domain, BV catalog # 6510-10) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 15 mg of rabbit IgG per ml of wet gel.
Product Highlights

•CONTENTS- Supplied as a 50% slurry in PBS with 0.02% sodium azide. •TECHNICAL SPECIFICATIONS- Parameter Description-Support Characteristics Paramagnetic, spherical, 6 % cross-linked agarose Ligand- Recombinant Protein G Particle Size- 75 – 150 µm Binding Capacity- Generally >15 mg rabbit IgG/ml wet beads Working Temperature- Room temperature Storage Solution- PBS w/0.02% NaN₃ Storage Temperature- 4 – 8 °C •FEATURES/APPLICATIONS- Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies. High affinity for Fc region of IgG antibodies from a variety of species.
Storage Temp

+4°C
Shipping

gel pack
Shelf Life

12 months
Datasheet
Notes

For research use only. Not to be used for human or animal treatment or consumption.
Conjugation

Magnetic beads

Gene target

Protein
Gene symbol

GPR88, GPR33, GPR173
Short name

Protein G
Technique

All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Magnetic bead separation is a quick, efficient, clean process that scientists use to replace filtration, centrifugation and separation techniques. Magnetic beads and particles are used as carriers of antigens, antibodies, catalyzers, proteins and nucleic acids, enabling action on cells, bacteria, viruses and other biological entities.
Label

Magnetic
Alternative name

Protein G Magnetic particles

Identity

HGNC:4539
Gene

GPR88
Long gene name

G protein-coupled receptor 88
Synonyms gene name
- G-protein coupled receptor 88
- G protein coupled receptor 88
GenBank acession
- AB042411
Locus

1p21.2
Discovery year

2000-06-02
Entrez gene record

54112
RefSeq identity
- NM_022049
Classification
- G protein-coupled receptors, Class A orphans
VEGA ID

OTTHUMG00000010981

Identity

HGNC:4489
Gene

GPR33
Long gene name

G protein-coupled receptor 33
Synonyms gene name
- G protein-coupled receptor 33, pseudogene
- G protein-coupled receptor 33 pseudogene
- G protein-coupled receptor 33
- G protein-coupled receptor 33 (gene/pseudogene)
GenBank acession
- AF045767
Locus

14q12
Discovery year

1997-06-12
Entrez gene record

2856
Pubmed identfication
- 9653656
- 15987686
RefSeq identity
- NM_001197184
Classification
- G protein-coupled receptors, Class A orphans
VEGA ID

OTTHUMG00000154921

Identity

HGNC:18186
Gene

GPR173
Long gene name

G protein-coupled receptor 173
Synonyms gene name
- G-protein coupled receptor 173
- G protein coupled receptor 173
Synonyms
- SREB3
GenBank acession
- AB040801
Locus

Xp11.22
Discovery year

2004-08-19
Entrez gene record

54328
Pubmed identfication
- 10833454
RefSeq identity
- NM_018969
Classification
- G protein-coupled receptors, Class A orphans
VEGA ID

OTTHUMG00000021596
Locus Specific Databases
- Global Variome shared LOVD

Name

Immunomagnetic Separation
Concept

Scope note: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
Tree numbers
- E02.095.437
- E05.200.500.363.441
- E05.242.363.441
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data