Protein A/G/L Magnetic Beads
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Catalog number
6547-1
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Price
Please ask
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Size
1ml
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Description
Reusable Protein A/G/L conjugated magnetic beads with ≥10 mg/ml binding capacity for antibody purification from multiple sources. Protein A/G/L Magnetic Beads are prepared by covalently coupling Recombinant fusion Protein A/G/L (contains thirteen IgG binding domains, BV catalog # 6540) to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 10 mg of human IgG per ml of wet gel.
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Product Highlights
• CONTENTS: Supplied as a 50% slurry in PBS with 0.02% sodium azide. • TECHNICAL SPECIFICATIONS: Support Characteristics- Paramagnetic, spherical, 6 % cross-linked agarose Ligand- Recombinant fusion Protein A/G/L Particle Size- 75 – 150 µm Binding Capacity- Generally >10 mg human IgG/ml wet beads Working Temperature- Room temperature Storage Solution- PBS w/0.02% NaN₃ Storage Temperature- 4 – 8 °C • FEATURES: Easy to use, high-binding capacity, non-adherent beads. Useful for immunoprecipitation and enrichment of IgG antibodies. High affinity for Fc region of IgG antibodies from a variety of species. Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind Protein A, Protein G and Protein L individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. .
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Storage Temp
+4°C
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Shipping
gel pack
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Shelf Life
12 months
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Notes
For research use only. Not to be used for human or animal treatment or consumption.
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Conjugation
Magnetic beads
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Gene target
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Short name
Protein A/G/L
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Technique
All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Magnetic bead separation is a quick, efficient, clean process that scientists use to replace filtration, centrifugation and separation techniques. Magnetic beads and particles are used as carriers of antigens, antibodies, catalyzers, proteins and nucleic acids, enabling action on cells, bacteria, viruses and other biological entities.
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Label
Magnetic
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Alternative name
Protein A/G/L Magnetic particles
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MeSH Data
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Name
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Concept
Scope note:
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
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Tree numbers
- E02.095.437
- E05.200.500.363.441
- E05.242.363.441
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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