Protein Carbonyl Content Assay Kit (Fluorometric)

• Catalog number
  K563-100
• Price
  Please ask
• Size
  100 assays

• Description
  The only, most sensitive kit in the market. Validated with human serum
• Summary
  • Detection method- Fluorometric (Ex/Em 485/535 nm) • Species reactivity- Mammalian
• Detection Method
  Fluorometric (Ex/Em 485/535 nm)
• Species Reactivity
  Mammalian
• Applications
  Quantification of carbonyl content as a putative biomarker for research in disease areas such as diabetes, multiple sclerosis, and Alzheimer’s diseas
• Sample Type
  Variable (protein containing samples)
• Features Benefits
  • Simple procedure; validated with human serum samples containing pmol of carbonylated protein
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Protein carbonylation occurs when chronically elevated blood sugar levels create a microenvironment for sugars to covalently react with amino acids forming non-enzymatic adducts. Oxidative stress, the excessive production of ROS, can also give rise to the production of protein carbonyls. Over time, these two processess create Advanced Glycation End-products (AGEs) with proteins. The major functional outcome of protein-reactive AGEs is reduced enzymatic activity. Soluble AGEs can also bind and activate the receptor of advanced glycation end-products (RAGE). Receptor-dependent outcomes lead to tissue dysfunction, oxidative stress, and activation of monocytes, endothelial cells and mesangial cells. In Alzheimer’s Disease (AD) protein carbonyls are a marker of oxidative stress in the brain and are elevated in serum and plasma proportional to the cognitive severity. BioVision’s Protein Carbonyl Content Assay Kit uses Fluoroscein-5-Thiosemicarbazide (FTC), a fluorescent probe which covalently reacts with oxidized residues (i.e Cysteine, Lysine, Arginine, Histidine and Aspartic Acid) on proteins. The protein carbonyl content is determined by the generation of a stable fluorometric signal (Ex/Em 485/535 nm) and compared to the protein concentration determined in the BCA Assay to quantitate nmoles of carbonyl/ mg protein. The kit is simple, requires no harsh chemicals, can quantitate carbonyls in serum or plasma and produces more reliable and reproducible results than the comparable colorimetric assays. It can detect carbonyl groups in samples with protein concentrations as low as 1 mg/ml!
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Protein   Carbonyl   Content   Kit   Fluorometric  
• Short name
  Protein Carbonyl Content Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Protein Carbonyl Content test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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