PKG Antibody

• Catalog number
  3452-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  PKG
• Target Alternative Name
  cGKI alpha CGKI cGKI beta cGMP dependent protein kinase 1alpha isozyme cGMP dependent protein kinase 1beta isozyme KGPB PRKG1 PR
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat, bovine
• Immunogen Sequence
  Synthetic peptide near the N-terminus of human PKG
• Accession
  ABQ59040
• Gene Id
  5592
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot,
• Application And Usages
  The antibody can be used for Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes 75 and 85 kDa PKG, corresponding to the molecular mass of two PKG isoforms.
• Description
  PKGs are cyclic GMP-dependent protein kinases (also designed cGKs) that are classified into two types, PKGI and PKGII. Studies have shown that cGKs and cyclic AMP-dependent kinases (cAKs) are highly homologous protein kinase families with similar substrate specificities. Phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility and platelet aggregation. However, recent studies using mice deficient in PKGs have shown that cGMP kinases regulate very specifically distinct pathways which are separate from those used by cyclic AMP kinases.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PKG  
• Gene symbol
  PRKG1
• Short name
  PKG Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  PKG (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9414
• Gene
  PRKG1
• Long gene name
  protein kinase cGMP-dependent 1
• Synonyms gene
  • PRKGR1B
  • PRKG1B
• Synonyms gene name
  • protein kinase, cGMP-dependent, type I
• Synonyms
  • PGK
  • PKG
  • PKG1
• Locus
  10q11.23-q21.1
• Discovery year
  1991-07-17
• Entrez gene record
  5592
• Pubmed identfication
  • 2792381
  • 1544322
• Classification
  • MicroRNA protein coding host genes
  • AGC family kinases
• VEGA ID
  OTTHUMG00000018248
• Locus Specific Databases
  • LRG_1135

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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