Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)
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Catalog number
K569-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Colorimetric (OD 450 nm) • Applications- Measurement of 6-PGD activity in purified/crude enzyme preparations and biological samples
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Detection Method
Colorimetric (OD 450 nm)
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Species Reactivity
Eukaryotes
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Applications
Measurement of PHGDH activity in purified/crude enzyme preparations and biological samples such as cell/tissue lysates
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Sample Type
Purified enzyme, tissue and cell lysates
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Features Benefits
• Simple, rapid & convenient assay to measure PHGDH Activity in biological samples • Includes Positive Control
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Phosphoglycerate Dehydrogenase (PHGDH: EC 1.1.1.95) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3-phosphohydroxyglycerate and the NADH-dependent reduction of α-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer. BioVision’s Phosphoglycerate Dehydrogenase Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm). The assay is simple, sensitive, high–throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbol
PHGDH
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Short name
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Phosphoglycerate Dehydrogenase (PHGDH) Activity test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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