Phospho-Chk2 (Thr68) Antibody

• Catalog number
  A1265-100
• Price
  Please ask
• Size
  100 µl

• Antibody Target
  CHEK2
• Target Alternative Name
  Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2, CHEK2
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, Mouse, Rat
• Immunogen Sequence
  Synthesized peptide derived from human Chk2 around the phosphorylation site of T68
• Accession
  O96017
• Gene Id
  11200
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Purification
  Affinity-chromatography using epitope-specific immunogen
• Application
  WB, IHC, ELISA
• Positive Control
  WB: HeLa cell lysate
• Application And Usages
  WB 1:500-1:2000; ELISA 1:20000; IHC 1:100-1:300
• Description
  Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Phospho-Chk2   Thr68  
• Short name
  Phospho-Chk2 (Thr68) Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  phosphorilated-Chk2 (Thr68) (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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