- Catalog numberbs-3721R-Cy5.5
- Product nameCHK2 (Thr68) Antibody, Cy5.5 Conjugated
- Size0.1ml
- PriceAsk For Price
- Short nameAnti-CHK2 Thr68
- Long nameCHK2 (Thr68) Polyclonal Antibody, Cy5.5 Conjugated
- CategoryConjugated Primary Antibodies
- ConjugationCy5.5
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenCHK2 Thr68
- SpecificityThis is a highly specific antibody against CHK2 Thr68.
- ModificationPhosphorylation
- Modification siteThr68
- ClonalityPolyclonal
- ClonePolyclonal antibody
- IsotypeImmunoglobulin G (IgG)
- Concentration1ug per 1ul
- Immunogen range50-90/543
- Subcellular locationNucleus
- SourceKLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68
- Gene ID number11200
- Swiss ProtO96017
- Tested applicationsIF(IHC-P)
- Recommended dilutionsIF(IHC-P)(1:50-200)
- CrossreactivityHuman, Mouse, Rat
- Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Antigen backgroundSerine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.
- Purification methodThis antibody was purified via Protein A.
- Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
- Excitation Emission675nm/694nm
- SynonymsCDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Checkpoint kinase 2; CHEK2
- Gene targetCHK2 Thr68 Conjugated
- Identity:HGNC:16627
- Gene:CHEK2
- Long gene name:checkpoint kinase 2
- Synonyms gene name:CHK2 (checkpoint, S.pombe) homolog, CHK2 checkpoint homolog (S. pombe)
- Synonyms:CDS1, CHK2HuCds1PP1425bA444G7
- Discovery year:2001-09-19
- Entrez gene record:11200
- Pubmed identification:9836640, 10097108
- RefSeq identity:NM_001005735
- VEGA ID:OTTHUMG00000151023
- Gene symbolCHEK2
- isotype filter
- Immunoglobulin G IgG
- LabelCy5.5
- label filter
- Cy5.5
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
- Alternative techniqueantibodies
Gene info
Locus Specific Databases:LRG_302