Caspase-5 Fluorometric Assay Kit

• Catalog number
  K122-200
• Price
  Please ask
• Size
  200 assays

• Description
  Convenient, Highly-Sensitive Assay
• Summary
  • Detection method- Fluorescence (Ex/Em 400/505 nm) • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
• Sample Type
  Cell and tissue lysates
• Features Benefits
  • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  6-12 months
• Background
  Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-5 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspase-5 and related caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

• Gene target
  Caspase-5   Fluorometric   Kit  
• Short name
  Caspase-5 Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  caspase-5 fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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