Phosphate Assay Kit (Fluorometric)

• Catalog number
  K420-100
• Price
  Please ask
• Size
  100 assays

• Description
  Rapid, simple and sensitive assay for phosphate measurement
• Summary
  • Detection method- Fluorescence (Ex/Em 535/587nm) • Species reactivity- Mammalian • Applications- Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
• Detection Method
  Ex/Em 535/587nm
• Species Reactivity
  Mammalian samples
• Applications
  Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use
• Features Benefits
  • Simple procedure; takes only ~2 hours • Fast and convenient • The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of its most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in a variety of samples. The newly designed and enhanced Phosphate Assay Kit provides a sensitive, easy and quick means of assessing Pi over a wide range of concentrations. In this assay, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate in the presence of a proprietary enzyme. The glucose-1-phosphate is specifically oxidized to generate a product that reacts with the PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, phosphorylase, etc. from a variety of samples. Unlike other commercially available assays, this assay is not affected by the presence of glucose in samples. This kit can determine phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Phosphate   Kit   Fluorometric  
• Short name
  Phosphate Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Phosphate test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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