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Description
for measuring PLP concentrations
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Summary
Pyridoxal 5’-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc. BioVision’s Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit provides a quick, specific and easy method for measuring PLP concentrations in a wide variety of samples. In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.
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Detection Method
Fluorescence detection (Ex/Em = 535/587 nm)
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Species Reactivity
All
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Applications
Analysis of Vitamin B6 in beverage and effects of Vitamin B6 intake on serum, Measurement of PLP in biological samples and beverages
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Sample Type
Biological fluids: serum, juices, etc., Mammalian tissue
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Features Benefits
Specific, Easy method for measuring PLP concentrations in a wide variety of samples., It can detect as low as 0.2 pmol/well of PLP in biological samples, Quick
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Pyridoxal 5’-phosphate (PLP), the active form of Vitamin B6 is a cofactor in a variety of metabolic reactions including transamination, decarboxylation, racemization, etc. Vitamin B6 comprises of pyridoxine, pyridoxamine and pyridoxal, which during metabolism is converted to the enzymatically active form namely PLP. Vitamin B6 is necessary for the synthesis of neurotransmitters, hemoglobin in red blood cells, fat metabolism etc. Vitamin B6 deficiency can lead to nervous disorders, peripheral neuropathy, anemia, seizures etc. BioVision’s Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit provides a quick, specific and easy method for measuring PLP concentrations in a wide variety of samples. In this assay, PLP reacts with a PLP-dependent enzyme thereby converting the substrate into an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 0.2 pmol/well of PLP in biological samples.
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Test
Biovision supplies other types of Assays as 1.
