p53 Nuclear Translocation Assay Kit (Cell-Based)

• Catalog number
  K961-50
• Price
  Please ask
• Size
  50 assays

• Summary
  • Detection method - Fluorescence Microscopy • Applications: • Visualize nuclear translocation and activation of p53 protein in mammalian cells. • Detection of increased cellular stress or DNA damage. • Screening for potential inhibitors that disrupt the interaction between p53 and MDM2
• Detection Method
  Fluorescence (Ex/Em = 360/460 nm)
• Species Reactivity
  Mammalian
• Applications
  Visualize nuclear translocation and activation of p53 protein in mammalian cells.
• Sample Type
  Adherent or suspension human cells
• Features Benefits
  • Simple procedure to visualize p53 nuclear translocation in ~ 3 hours • Fast and contains Nutlin-3 that serves as a control to induce p53 translocation from the cytoplasm to the nucleus.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  p53 is a tumor suppressor gene which encodes a transcription factor that controls cell’s destiny. This gene gets activated when cells are exposed to increased cellular stress, or DNA damage. The levels of p53 are elevated when post-translational modifications occur, which block its sequestration and/or ubiquitination by its destabilizer, Mouse Double Minute 2 Homolog (MDM2) which in turn is a transcriptional target of p53. Due to the three putative nuclear localization signals (NLSs) on its C-terminus, some of the activated p53 protein translocates into the nucleus and activate genes that induce cell cycle arrest, senescence, or apoptosis. The p53 gene is frequently mutated in cancer cells, and about 50% of cancers show p53 mutations, resulting in loss of its functions. The loss of p53 functionality can lead to dysregulation of many biological processes in cells, such as metabolic pathways, cellular homeostasis, cell movement, apoptosis, etc. BioVision’s p53 Nuclear Translocation Assay Kit (Cell-Based) provides an easy and complete assay kit to visualize the activation and nuclear translocation of p53 in human cells. This assay kit uses BioVision’s specific and sensitive human p53 antibody and a p53 secondary antibody to visualize the localization of p53 in fixed common human cells along with DAPI, a fluorescent stain, for nuclear staining. The kit includes Nutlin-3, a potent selective inhibitor that disrupts the protein-protein interaction between p53 and MDM2. Nutlin-3 serves as a control to induce p53 translocation from the cytoplasm to the nucleus
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  p53   Translocation   Kit   Based  
• Short name
  p53 Translocation Assay Kit ( -Based)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  p53 Nuclear Translocation test reagent (cellular-Based)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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