Phospho-p38 MAPK (Thr¹⁸⁰+Tyr¹⁸²) Translocation Assay Kit (Cell-Based)

• Catalog number
  K965-100
• Price
  Please ask
• Size
  100 assays

• Description
  A cell-based assay kit that detect the nuclear translocation of activated p38 in fixed mammalian cells.
• Summary
  • Detection method- fluorescence microscope capable of measuring EX/EM at 550 nm and equipped with UV filter for DAPI • Species reactivity- Human, mouse, rat • Application- • Detection of nuclear translocation of phosphorylated-p38 in mammalian cells
• Detection Method
  Fluorescence microscope capable of measuring EX/EM at 550 nm and equipped with UV filter for DAPI
• Species Reactivity
  Human, mouse, rat
• Applications
  Detection of nuclear translocation of phosphorylated-p38 in mammalian cells
• Sample Type
  Adherent or suspension mammalian cell cultures
• Features Benefits
  Detection of nuclear translocation of phosphorylated-p38 in mammalian cells Screening effectors of p38 MAPK signaling pathway Detection of cellular stress, DNA damage, heat shock, and pro-inflammatory stimuli
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Mitogen-activated protein kinases (MAPKs) are proline-directed serine and threonine protein kinases that regulate numerous physiological and pathophysiological cell responses. p38 MAPKs belong to a class that plays an important role during inflammatory processes, and their expression is upregulated by inflammatory and stress stimuli (cytokines, ultraviolet irradiation, osmotic and heat shock). p38 MAPKs are also involved in autophagy, apoptosis, cell differentiation, and are implicated in the regulation of inflammatory mediators levels such as tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2). Accumulating evidence suggests that p38 MAPKs play an important role in arthritis, and inflammation of the liver, kidney, brain, and lung. Diseases induced by chronic inflammation, including gastritis, colitis, dermatitis, rheumatoid arthritis, pulmonary diseases and type II diabetes affect millions of people every year. Therefore, p38 is an important target in better understanding of inflammation and development of novel treatments. p38 MAPK is activated following phosphorylation at Thr180/Tyr182 by upstream MKK6 and MKK3 kinases. While in quiescent cells p38 localizes to both nucleus and cytosol, stress induced conformational changes within the active site result in selective translocation of activated-p38 into the nucleus. Thus, the selective nuclear accumulation of p38 could be a mechanism to facilitate the phosphorylation of p38 MAPK nuclear targets. BioVision’s Phospho-p38 MAPK (Thr180+Tyr182) Translocation Assay Kit (Cell-Based) provides a valuable tool to detect the nuclear translocation of activated p38 in fixed mammalian cells.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Phospho-p38   MAPK   Thr¹⁸⁰+Tyr¹⁸²   Translocation   Kit   Based  
• Short name
  Phospho-p38 MAPK (Thr¹⁸⁰+Tyr¹⁸²) Translocation Assay Kit ( -Based)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  phosphorilated-p38 MAPK (Thr¹⁸⁰andTyr¹⁸²) Translocation test reagent (cellular-Based)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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