Methylglyoxal Assay Kit (Colorimetric)
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Catalog number
K500-100
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Price
Please ask
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Size
100 assays
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Description
First kit in the market that can detect Methylglyoxal in samples
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Summary
• Detection method- Absorbance (OD 450 nm) • Species reactivity- Eukaryotes • Applications- This Kit provides a simple in vitro assay for detecting Methylglyoxal levels in biological samples.
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Detection Method
Absorbance (OD 570 nm)
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Species Reactivity
Eukaryotes
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Applications
Measurement of Methylglyoxal in food products
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Sample Type
• Food Products: Manuka Honey, etc.
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Features Benefits
• Simple procedure; takes ~ 2 hours • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable • Useful for the estimation of Methylglyoxal in biological samples
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Methylglyoxal ( mg, 2-oxopropanal, pyruvic aldehyde) is a highly reactive dicarbonyl compound formed during glycolysis and sugar fermentation. It is an important precursor of advanced glycation end products (AGEs). Endogenous mg is present in many food items and beverages. For example, high levels of mg have been reported in Manuka Honey and some soft drinks. Additionally, cooking and some storage techniques can induce the synthesis of mg, such as coffee brewing, and bread toasting. It has been shown that mg has antimicrobial activity against some bacterial strains, including Streptococcus, H. pylori, E. coli and other bacteria resistant to standard antibiotic treatments. BioVision’s Methylglyoxal Assay Kit (Colorimetric) enables the detection of mg using a set of engineered enzymes and a chromophore. The reduced chromophore, final product of the assay, produces a stable signal, which can be easily quantified at 450 nm using a microplate reader and its signal is directly proportional to the amount of mg in samples. The assay is simple, specific, reproducible, and can detect as low as 0.5 nmol/well of mg in a 100 µl reaction.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Methylglyoxal Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Methylglyoxal test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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