MagPureTM 96-well Plasmid Purification Kit

• Catalog number
  K1477-1
• Price
  Please ask
• Size
  1 Plate

• Description
  Magnetic Beads based purification
• Summary
  Plasmid is purified using magnetic beads in a 96-well plate
• Detection Method
  Purification of plasmids using magnetic beads in a 96-well plate
• Applications
  Parallel purification of plasmids from 96 samples
• Sample Type
  Bacterial cells
• Features Benefits
  Highly efficient Magnetic particles,Highly pure DNA, high yield,Many downstream applications such as DNA Sequencing, Restriction Enzyme digestion etc.,Rapid, easy and convenient
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  BioVision's MagPureTM 96-well Plasmid Purification Kit combines the efficiency of magnetic bead technology with alkaline-SDS lysis of bacterial cells to purify high quality plasmid DNA. The kit is used to extract and purify plasmids using a 96-well Lysate Clearance Plate and magnetic particles. The cleared lysate obtained using the 96-well clearance plate in the first step is further purified using magnetic beads. The proprietary magnetic particles bind to DNA or RNA avidly, but reversibly under optimal conditions. The new 96-well Lysate Clearance Plate removes the need of time-consuming centrifugation to clear bacterial alkaline lysates. Proteins and other contaminants are removed and the nucleic acids can then be easily eluted with deionized water or low salt buffer. The purified plasmid can be used directly for automated fluorescent DNA sequencing and other standard molecular biology techniques such as restriction enzyme digestion. 1 ml of overnight culture in LB medium typically yields 10-15 μg high-copy plasmid DNA.
• Data Sheet Link
• Kit
  Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
• Test
  Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.

• Gene target
  MagPureTM   Kit  
• Short name
  MagPureTM 96- Plasmid Purification Kit
• Technique
  Purification, plasmid, plasmids in 1, purified
• Alternative name
  MagPureTM 96-well Plasmid purification reagent
• Alternative technique
  purifications, plasmids, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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