Plasmid DNA extraction and purification kit
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Catalog number
K1445-100
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Price
Please ask
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Size
100 test
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Description
Plasmid DNA extraction and purification
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Summary
Plasmid DNA extraction and purification kit allows extracting and purifying plasmid DNA from 2.0~5.0 mL of bacteria culture. The typical yield is 10~20 µg of high quality plasmid DNA, and the typical OD260/280 is between 1.75 and 1.85.
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Applications
Extraction and purification of plasmid DNA from bacteria culture.
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Sample Type
bacteria culture
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Features Benefits
Typically yields 10~20 µg of high quality plasmid DNA, Purified DNA can be directly used for enzymatic digestion, sequencing and transformation., Extraction and purification of plasmid DNA from bacteria culture.
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Storage Conditions
RT and 2-8°C as described in the datasheet
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Shipping Conditions
RT
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Shelf life
12 months
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Background
Plasmid DNA extraction and purification kit with proprietary separation and buffer systems, allows extracting and purifying plasmid DNA from 2.0~5.0 mL of bacteria culture. The typical yield is 10~20 µg of high quality plasmid DNA, and the typical OD260/280 is between 1.75 and 1.85. Purified plasmid DNA can be directly used for a variety of molecular biology applications such as enzymatic digestion, sequencing and transformation. The kit will work with a 48 well round bottom plates if a special magnetic frame is used. The kit can also be used with a variety of automatic nucleic acid extraction instruments or workstations.
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Kit
Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Plasmid DNA extraction purification kit
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Technique
Purification, plasmid, dna, plasmids in 1, purified
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Alternative name
Plasmid Desoxyribonucleic acid extraction and purification reagent
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Alternative technique
purifications, plasmids, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
Product images
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