Lysine Assay Kit (Fluorometric)

• Catalog number
  K2005-100
• Price
  Please ask
• Size
  100 assays

• Description
  Market's first and only kit for enzymatic quantification of L-lysine levels in various biological samples
• Summary
  BioVision’s Lysine Assay Kit allows the highly sensitive quantification of L-lysine levels in various biological samples. The assay is based on the selective enzymatic metabolism of lysine, yielding an oxidized intermediate which reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by the physiological concentration of other amino acids and is high-throughput adaptable. The kit can detect less than 5 μM Lysine in samples.
• Detection Method
  Fluorecence Ex/Em = 538/587 nm
• Species Reactivity
  All
• Applications
  Estimation of L-Lysine concentration in various biological samples
• Sample Type
  Soft tissue homogenates (i.e. liver, brain, etc.), Cultured cell lysates (adherent or suspension cells) or cell culture growth/fermentation medium, Human or animal biological fluids (plasma, serum, etc.)
• Features Benefits
  Allows the highly sensitive quantification of L-lysine levels in various biological samples., Market's first and only kit for enzymatic quantification of L-lysine levels in various biological samples., The assay is not affected by the physiological concentration of other amino acids., The kit can detect less than 5 μM Lysine in samples., High-throughput adaptable.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Lysine is one of the 20 naturally-occuring proteogenic amino acids and an essential dietary amino acid. In addition to its indispensable nutritional role, lysine is a critical component of the fibrous connective tissue proteins such as collagen and elastin. Lysine residues in fibril proteins are subjected to post-translational oxidation, forming reactive aldehydes that crosslinks adjacent polypeptide strands. Post-translational methylation and acetylation of the ε-amino moiety in histone lysine residues is a primary mechanism of epigenetic transcriptional regulation. Lysine is also required for the synthesis of carnitine, which assists in mitochondrial fatty acid oxidation. Dietary lysine deficiency, frequent in impoverished populations with limited access to high-quality protein sources, results in anemia and the suppression of growth and immune function. Additionally, lysine deficiency severely impairs the ability to cope with stress-induced anxiety or recovery from injuries. BioVision’s Lysine Assay Kit allows the highly sensitive quantification of L-lysine levels in various biological samples. The assay is based on the selective enzymatic metabolism of lysine, yielding an oxidized intermediate which reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by the physiological concentration of other amino acids and is high-throughput adaptable. The kit can detect less than 5 μM Lysine in samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Lysine   Kit   Fluorometric  
• Short name
  Lysine Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Lysine test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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