Homocysteine Assay Kit (Fluorometric)

• Catalog number
  K531-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple, Highly Sensitive, HTS adaptable assay to measure Homocysteine in a variety of biological samples.
• Summary
  • Detection method- Fluorescence (Ex/Em = 658/708 nm) • Species reactivity- Mammalian • Applications- Estimation of Homocysteine in various biological samples. • Detection limit/sensitivity: Can detect as little as 5 µM of Homocysteine
• Detection Method
  Fluorescence (Ex/Em = 658/708 nm)
• Species Reactivity
  Mammalian
• Applications
  Estimation of Homocysteine in various biological samples
• Sample Type
  Serum, plasma
• Features Benefits
  • Simple, sensitive and HTP adaptable Protocol • Specific for Homocysteine without interference from other thiol based amino acids • This kit can detect as little as 5 µM of Homocysteine in a variety of biological samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Homocysteine is a non-proteogenic amino acid synthesized intracellularly by removal of the N-methyl group from the essential amino acid methionine. Homocysteine is exported from cells into the blood, where it exists mainly as an oxidized disulfide species, either as a dimer or bound to cysteine residues of serum proteins. The reduced form of homocysteine (‘free’ homocysteine) can be metabolized into cysteine via the transsulfuration pathway; however, it can also undergo intramolecular cyclization, forming the highly reactive pro-oxidant homocysteine thiolactone. Subsequent N-homocysteinylation of protein lysine residues by the reactive thiolactone disrupts protein conformation, leading to formation of cytotoxic protein aggregates. Homocysteinylated proteins may also act as autoantigens, triggering arterial inflammation and atherosclerosis. Elevated plasma homocysteine concentration is a clinical biomarker for increased risk of cardiovascular disease, ischemic stroke and myocardial infarction. Severely elevated homocysteine levels (hyperhomocysteinemia) are correlated with a 4-fold increase in mortality due to heart attack and a 16-fold increase in the likelihood of recurrent stroke. BioVision’s Homocysteine Assay Kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine-selective enzyme, generating an intermediate product. The intermediate reacts with a probe solution to form a stable fluorophore that emits in the far-red spectrum (Ex/Em = 658/708 nm). The assay is not affected by physiological concentrations of other biological thiols (such as cysteine, methionine and glutathione), is high-throughput adaptable and can detect as low as 5 μM homocysteine.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Homocysteine   Kit   Fluorometric  
• Short name
  Homocysteine Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Homocysteine test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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