HDAC-5 Inhibitor Screening Kit (Fluorometric)
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Catalog number
K171-100
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Price
Please ask
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Size
100 assays
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Description
Useful for the identification of potential HDAC-5 inhibitors
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Summary
• Detection method- Fluorescence (Ex/Em = 380/460 nm) • Applications: - Screening potential inhibitors of Histone Deacetylase 5 - Characterizing/studying HDAC5 inhibitors
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Detection Method
Fluorescence (Ex/Em = 380/460 nm)
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Applications
• Screening potential inhibitors of Histone Deacetylase 5 • Characterizing/studying HDAC5 inhibitors in plasma samples
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Features Benefits
• Simple and reliable test to screen/study/characterize HDAC5 inhibitors • High-throughput compatible • Includes Inhibitor Control (Trichostatin A)
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Storage Conditions
-80°C
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Shipping Conditions
dry ice
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Shelf life
12 months
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Background
Histone Deacetylase 5 or HDAC-5 belongs to Class II Histone deacetylase family. It represses transcription by deacetylating lysine residues on the N-terminal part of histones H2A, H2B, H3 and H4. This epigenetic repression caused by HDAC-5, plays a role in transcriptional regulation, cell cycle progression and development. It interacts with myocyte enhancing factor (MEF-2) proteins that are associated with colon cancer, causing the repression of MEF-2 dependent gene expression. HDAC-5 is also involved in epigenetic regulation of ESR1 expression in breast cancer. HDAC-5 inhibition induces reactive oxygen species (ROS) production, ultimately leading to apoptotic cell death as well as mechanisms of mitochondrial quality control processes such as mitophagy and mitobiogenesis. Therefore, HDAC-5 inhibition in cancer cells significantly reduces tumor size due to apoptotic cell death of cancer cells (Hendrick, et al, Oncogene. 2017 Aug 24; 36(34):4859-4874), which makes HDAC-5 an attractive target for anti-cancer therapy. BioVision’s HDAC-5 Inhibitor Screening Kit is a plate-based fluorometric kit for screening potential HDAC-5 inhibitors. The principle of the kit is based on the deacetylation of an acetylated substrate by HDAC-5. The deacetylated product is then cleaved by a developer to release a highly fluorescent moiety with Ex/Em= 380/460 nm. Trichostatin A, a well-known HDAC inhibitor is provided as inhibitor control.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
HDAC4, HDAC9, HDAC5, MIR1302-5, PIRC22, PIRC23, PIRC20, PIRC21, HTR6, IGKV3OR2-5
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Short name
HDAC-5 Inhibitor Screening Kit (Fluorometric)
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Alternative name
HDAC-5 suppressor detection reagent (fluorescence)
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 4
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Synonyms gene
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Synonyms gene name
- brachydactyly-mental retardation syndrome
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIA
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 9
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Synonyms
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GenBank acession
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Locus
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Discovery year
2002-09-06
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Entrez gene record
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Pubmed identfication
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Classification
- Histone deacetylases, class IIA
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 5
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Synonyms
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIA
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Product images
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