HDAC Inhibitor Drug Screening Kit (Fluorometric)

• Catalog number
  K340-100
• Price
  Please ask
• Size
  100 assays

• Description
  Non-radioactive, Two Easy Steps, HTS
• Summary
  • Detection method- Fluorescence (Ex/Em 350-380/440-460 nm) • Species reactivity- Mammalian • Applications- Screening of compounds for HDAC inhibition by analyzing HDAC activity
• Sample Type
  Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Features Benefits
  • Simple two-step procedure • Fast fluorescence-based method that eliminates radioactivity, extractions, or chromatography • The assay is well suited for high throughput screening applications.
• Storage Conditions
  -80°C
• Shipping Conditions
  dry ice
• Shelf life
  12 months
• Background
  Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells. However, screening of compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The new HDAC Inhibitor Drug Screening Kit provides a fast, fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, your inhibitor candidates are mixed with HeLa Nuclear Extract and HDAC fluorometric substrate, which comprises an acetylated lysine side chain. Deacetylation of the substrate sensitizes the substrate, so that, in the second step, treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  HDAC   Inhibitor   Drug   Screening   Kit   Fluorometric  
• Gene symbol
  HDAC4, HDAC9
• Short name
  HDAC Inhibitor Drug Screening Kit (Fluorometric)
• Alternative name
  HDAC suppressor Drug detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:14063
• Gene
  HDAC4
• Long gene name
  histone deacetylase 4
• Synonyms gene
  • BDMR
• Synonyms gene name
  • brachydactyly-mental retardation syndrome
• Synonyms
  • KIAA0288
  • HDAC-A
  • HDACA
  • HD4
  • HA6116
  • HDAC-4
• GenBank acession
  • AB006626
• Locus
  2q37.3
• Discovery year
  2000-11-28
• Entrez gene record
  9759
• Pubmed identfication
  • 10206986
  • 10220385
  • 20691407
• RefSeq identity
  • NM_006037
• Classification
  • Histone deacetylases, class IIA
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133344

Gene info

• Identity
  HGNC:14065
• Gene
  HDAC9
• Long gene name
  histone deacetylase 9
• Synonyms
  • KIAA0744
  • HDAC
  • MITR
  • HD7
  • HDAC7B
• GenBank acession
  • AF124924
• Locus
  7p21.1
• Discovery year
  2002-09-06
• Entrez gene record
  9734
• Pubmed identfication
  • 10523670
  • 10487760
• Classification
  • Histone deacetylases, class IIA
• VEGA ID
  OTTHUMG00000152487

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