Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric)

• Catalog number
  K2018-100
• Price
  Please ask
• Size
  100 assays

• Description
  for the measurement of GA3P in biological samples
• Summary
  Glyceraldehyde 3-phosphate (GA3P), also known as triose phosphate or 3-phosphoglyceraldehyde is an important metabolite that occurs as an intermediate central to several biochemical pathways such as glycolysis and gluconeogenesis. It is formed during glycolysis by the breakdown of fructose-1,6-biphosphate by the enzyme fructose biphosphate aldolase. It also occurs as a byproduct in tryptophan biosynthesis pathway or as a reactant in thiamine biosynthesis pathway. In plants, GA3P is the product of the Calvin Cycle and is the starting molecule for the synthesis of other carbohydrates. BioVision’s Glyceraldehyde 3-Phosphate assay kit is a robust, one step plate based assay for the measurement of GA3P in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Species Reactivity
  All
• Applications
  Quantification of Glyceraldehyde 3-phosphate
• Sample Type
  Cell lysate, Tissue lysate
• Features Benefits
  Robust, Simple, One step plate based assay for the measurement of GA3P in biological samples
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Glyceraldehyde 3-phosphate (GA3P), also known as triose phosphate or 3-phosphoglyceraldehyde is an important metabolite that occurs as an intermediate central to several biochemical pathways such as glycolysis and gluconeogenesis. It is formed during glycolysis by the breakdown of fructose-1,6-biphosphate by the enzyme fructose biphosphate aldolase. It also occurs as a byproduct in tryptophan biosynthesis pathway or as a reactant in thiamine biosynthesis pathway. In plants, GA3P is the product of the Calvin Cycle and is the starting molecule for the synthesis of other carbohydrates. BioVision’s Glyceraldehyde 3-Phosphate assay kit is a robust, one step plate based assay for the measurement of GA3P in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Glyceraldehyde   3-Phosphate   Kit   Fluorometric  
• Short name
  Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Glyceraldehyde 3-Phosphate test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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