GFAP, Human Recombinant

• Catalog number
  P1364-10
• Price
  Please ask
• Size
  10 µg

• Synonyms
  Glial Fibrillary Acidic Protein, GFAP
• Alternative_names
  Glial Fibrillary Acidic Protein, GFAP
• Description
  A class-III intermediate filament that distinguishes astrocytes from other glial cells during the development of the central nervous system
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS-PAGE
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.25.
• Reconstitution Instructions
  Reconstitute in sterile deionized water to a concentration of 100 µg/ml.
• Background Information
  Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament (IF) protein which belongs to the intermediate filament family. GFAP is expressed in numerous cell types of the central nervous system (CNS), ependymal cells and phosphorylated by PKN1. GFAP, a class-III intermediate filament, is a cell-specific marker during the development of the central nervous system and distinguishes astrocytes from other glial cells. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood.
• Amino acid sequence
  MGSSHHHHHHSSGLVPRGSHMLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used on humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GFAP  
• Gene symbol
  GFAP
• Short name
  GFAP, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  GFAP, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4235
• Gene
  GFAP
• Long gene name
  glial fibrillary acidic protein
• Synonyms
  • FLJ45472
• Synonyms name
  • intermediate filament protein
• GenBank acession
  • S40719
• Locus
  17q21.31
• Discovery year
  1989-12-07
• Entrez gene record
  2670
• Pubmed identfication
  • 9693047
• RefSeq identity
  • NM_002055
• Classification
  • Intermediate filaments Type III
• VEGA ID
  OTTHUMG00000179866

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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