Rabbit Anti Rat Gfap Antibody-FITC
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Catalog numberCSB-PA009369LC01RA_100ug
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Price:
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Size100ug
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Long nameRabbit Antibody against Rat Gfap, FITC
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Synonym nameGfap Rat Antibody
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Species reactivityRat
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Tested applications recommended dilutionsELISA
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ConjugationFITC
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Antibody isotypeIgG
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UniProt IDP47819
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FormatLiquid, diluted in buffer
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Buffer compositionPreservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity>95%
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Purification methodProtein G purified
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Antibody categoryPolyclonal Antibodies
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Immunogen typeRecombinant Rat Glial fibrillary acidic protein (1-430AA)
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Immunogen speciesRat
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AliasGlial fibrillary acidic protein,GFAP,Gfap
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BackgroundGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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ClonalityPolyclonal antibody
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Shipping and storageGfap Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigenGlial fibrillary acidic protein
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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AboutRabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameOryctolagus cuniculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolGFAP
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Short nameAnti-Gfap
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TechniqueAntibody, Rabbit, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein
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HostRabbit, Rabbits
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LabelFITC
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SpeciesRat, Rats
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Alternative nameGfap Antibody,FITC conjugated
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Alternative techniqueantibodies, rabbit-anti, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameglial fibrillary acidic protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1989-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Intermediate filaments Type III
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data