Gelatin, Fluorescein (FITC) Conjugate

• Catalog number
  M1303-1
• Price
  Please ask
• Size
  1 mg

• Description
  An ideal substrate for detection of gelatin binding proteins and live cell staining. Collagen is a major component of the extracellular matrix in vertebrates and it constitutes approximately 25% of total protein. It plays an important role in cell adhesion and migration. Specific collagen receptors, fibronectin and a number of other proteins involved in cell–cell and cell–surface adhesion have been demonstrated to bind collagen and gelatin (denatured collagen). Gelatin-FITC is labeled with a fluorophore, fluorescein Isothiocyanate (FITC) and the conjugate is a highly quenched substrate for gelatinases/collagenases. It can be digested by gelatinases, collagenases and other matrix degrading metalloproteases, releasing brightly fluorescent peptides (Ex/Em 490/520 nm). The increase in fluorescence upon digestion is proportional to the proteolytic activity of the enzyme under investigation. Gelatin-FITC can also be used to detect gelatin binding proteins and live cell staining.
• Product Highlights
  FORM: Lyophilized FORMULATION: 5 mg/ml protein in TBS STORAGE CONDITIONS: Stable for one year at -20°C APPLICATIONS/BENEFITS: • Substrate for detection of Gelatinases/Collagenase and other gelatin degrading enzymes •To study gelatin binding proteins • Live cell staining
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Properties
  This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Gelatin   Fluorescein   Conjugate  
• Short name
  Gelatin, Fluorescein (FITC) Conjugate
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Gelatin, Fluorescein (fluorecein) Conjugate
• Alternative technique
  fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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