BrdU (bromodeoxyuridine) FITC Conjugate

Products_type

Antibody
Products_short_name

[BrdU (bromodeoxyuridine)]
Clonality

Monoclonal
Clone

[MoBU-1]
Reactivity

BrdU Incorporated Cells
Purity

Protein A/G Chromatography
Form

Provided as solution in phosphate buffered saline with 0.08% sodium azide
Storage_stability

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Tested_application

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Properties

This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data