Gelatin, Fluorescein (FITC) Conjugate

• Catalog number
  M1303-5
• Price
  Please ask
• Size
  5 x 1 mg

• Description
  An ideal substrate for detection of gelatin binding proteins and live cell staining. Collagen is a major component of the extracellular matrix in vertebrates and it constitutes approximately 25% of total protein. It plays an important role in cell adhesion and migration. Specific collagen receptors, fibronectin and a number of other proteins involved in cell–cell and cell–surface adhesion have been demonstrated to bind collagen and gelatin (denatured collagen). Gelatin-FITC is labeled with a fluorophore, fluorescein Isothiocyanate (FITC) and the conjugate is a highly quenched substrate for gelatinases/collagenases. It can be digested by gelatinases, collagenases and other matrix degrading metalloproteases, releasing brightly fluorescent peptides (Ex/Em 490/520 nm). The increase in fluorescence upon digestion is proportional to the proteolytic activity of the enzyme under investigation. Gelatin-FITC can also be used to detect gelatin binding proteins and live cell staining.
• Product Highlights
  FORM: Lyophilized FORMULATION: 5 mg/ml protein in TBS STORAGE CONDITIONS: Stable for one year at -20°C APPLICATIONS/BENEFITS: • Substrate for detection of Gelatinases/Collagenase and other gelatin degrading enzymes •To study gelatin binding proteins • Live cell staining
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Properties
  This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Gelatin   Fluorescein   Conjugate  
• Short name
  Gelatin, Fluorescein (FITC) Conjugate
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Gelatin, Fluorescein (fluorecein) Conjugate
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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