EZScreen™ Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)

  • Catalog number
    K941-400
  • Price
    Please ask
  • Size
    400 assays
  • Description
    Sensitive Assay, HTS
  • Summary
    • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit detects ALT with a detection limit of 0.1 µU per well.
  • Detection Method
    Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
  • Species Reactivity
    Eukaryotes
  • Applications
    • Evaluation of pentose phosphate pathway
  • Sample Type
    Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc.
  • Features Benefits
    • Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
  • Test
    Biovision supplies other types of Assays as 1.
  • Gene target
  • Short name
    EZScreen™ Alanine Aminotransferase (ALT SGPT) Activity Assay Kit (384- )
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    EZScreen™ Alanine Aminotransferase (Alanine aminotransferase and SGPT) Activity test reagent (384-Well)
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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