Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit

• Catalog number
  K752-100
• Price
  Please ask
• Size
  100 assays

• Description
  Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit detects ALT with a detection limit of 10 mU per well.
• Applications
  • Evaluation of pentose phosphate pathway
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc.
• Features Benefits
  • Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Alanine   Aminotransferase   ALT   SGPT   Activity   Fluorometric   Kit  
• Short name
  Alanine Aminotransferase (ALT SGPT) Activity Colorimetric/Fluorometric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Alanine Aminotransferase (Alanine aminotransferase and SGPT) Activity colorimetry/fluorescence test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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