EZClick™ Total Phospholipid Assay Kit (Cell-Based)

• Catalog number
  K537-100
• Price
  Please ask
• Size
  100 assays

• Description
  Microplate-based assay to detect total phospholipid content using metabolic labeling techniques
• Summary
  • Detection method- Fluorescence (Ex/Em= 494/521 nm) • Sample type- Adherent and suspension cells • Species reactivity- Mammalian • Applications- This assay provides a convenient and accurate procedure to measure de novo Protein synthesized in biological samples.
• Detection Method
  Fluorescence (Ex/Em= 494/521 nm)
• Species Reactivity
  Eukaryotes
• Applications
  This assay provides a convenient and accurate procedure to measure totla phopholipid content in cell cultures
• Sample Type
  Adherent and suspension cells
• Features Benefits
  Simple, fast, does not require lengthy incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phospholipids are major component of the bilayers of all plasma membranes. A single phospholipid molecule consists of a phosphate group on one (“head”), and two side-by-side chains of fatty acids that make up the "tails”. The phosphate head groups can be modified with organic molecules such as Choline (Cho). Cho-containing phospholipids (Phosphatidylcholines; PC) are critical for structural membrane integrity, cellular metabolism and signaling either as individual molecules or precursors of secondary messengers. Changes in global synthesis of Cho-containing phospholipids are an essential parameter in analysis of cellular response to both, physiological and pathological conditions, environmental stress, or drug treatment. To date, phospholipids biochemistry, cell biology and metabolism remain obscure, due to limited methods for their direct cellular visualization. BioVision’s EZClick™ Global Phospholipid Synthesis Assay Kit offers a simple and robust method to label and visualize newly synthesized phospholipids in vivo. Based on the metabolic incorporation of the choline analogs directly into their structure, modified phospholipid molecules can be detected with high sensitivity and spatial resolution by click chemistry with azide-containing dyes (Ex/Em= 494/521 nm). This kit enables quantitative analyses of global biosynthesis/turnover of Cho-containing phospholipids in cells. Cells show strong incorporation of Cho analogs into all classes of phospholipids that can be assayed by microplate reader and fluorescence microscope. The kit provides sufficient materials for 100 assays.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  EZClick™   Phospholipid   Kit   Based  
• Short name
  EZClick™ Phospholipid Assay Kit ( -Based)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  EZClick™ Total Phospholipid test reagent (cellular-Based)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell, total

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