Cell Transformation Assay Kit (Fluorometric)
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Catalog number
K922-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive and Quantitative (Fluorometric), HTP adaptable Assay to measure Cell Transformation
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Summary
• Detection method - Fluorescence (Ex/Em = 480/530 nm) • Applications: Measure cell transformation in response to stimuli. - Screen, study, or characterize compounds that influence cell transformation. - HTP adaptable
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Detection Method
Fluorescence (Ex/Em = 480/530 nm)
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Species Reactivity
Mammalian cells
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Applications
• Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation
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Sample Type
Adherent or Suspension Cells
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Features Benefits
• Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name
Transformation Assay Kit (Fluorometric)
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Technique
Assay, assays
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Host
Assay
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Alternative name
cellular Transformation test reagent (fluorescence)
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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Product images
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