Cell Transformation Assay Kit (Colorimetric)

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• Catalog number
  K921-100
• Price:

  617.04 USD

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• Size
  100 assays

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• Description
  Highly Sensitive and Quantitative (Colorimetric), HTP adaptable Assay to measure Cell Transformation
• Summary
  • Detection method - Colorimetric (OD 450 nm) • Applications: Measure cell transformation in response to stimuli. - Screen, study, or characterize compounds that influence cell transformation. - HTP adaptable
• Detection Method
  Colorimetric (OD 450 nm)
• Species Reactivity
  Mammalian cells
• Applications
  • Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation
• Sample Type
  Adherent or Suspension Cells
• Features Benefits
  • Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

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• Gene target
  Transformation   Kit  
• Short name
  Transformation Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  cellular Transformation test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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