Calcium Assay Kit (Fluorometric)

• Catalog number
  K409-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Fluorescence (500/530 nm) • Species reactivity- Eukaryotes • Limit of detection < 0.1 µM
• Detection Method
  Fluorescence (500/530 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Measurement of Calcium concentration in biological samples • Analysis of the effects of effectors/synthetic agents/stimuli on Calcium signaling
• Sample Type
  • Biological Fluids: Serum, Plasma, etc.
• Features Benefits
  • Simple procedure; takes less than 30 minutes • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Calcium (Ca, Atomic Number: 40), is an essential element for all living organisms. In human, Calcium ion (Ca2+) is released from bone to bloodstream and its level in serum is tightly controlled (8.4-11.4 mg/dL). Ca2+ acts as second messenger and plays many biological roles in signal transduction, gene expression, muscle contraction, synaptic plasticity and hormone release. Recent studies have found that dysregulation of calcium homeostasis leads to health problems, such as Alzheimer’s Diseases and Parkinson’s Disease, etc. BioVision’s Calcium Fluorometric Assay kit provides an easy and quantitative method to measure Calcium in various biological fluids. Low concentration of N,N,N’,N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) is included in the assay and used to pre-treat samples to eliminate interference from other di-valent metals such as Zinc. The assay is based on the ability of our proprietary probe that binds specifically to Calcium cations generating a high fluorescence signal. The fluorescence is directly proportional to the amount of calcium, which can be quantified by measuring the generated fluorescence (Ex/Em= 500/530 nm) using a microplate reader. This assay kit provides an add-and-read, non-radioactive, and high-throughput adaptable assay suitable for Calcium detection. The assay is rapid (less than 10 min) and sensitive (Limit of Detection:
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Calcium   Kit   Fluorometric  
• Short name
  Calcium Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Calcium test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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