BPTF bromodomain (2796-2907 aa) (GST-tagged), Human recombinant
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Catalog number
7662-100
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Price
Please ask
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Size
100 ug
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Synonyms
BPTF bromodomain (2796-2907 aa) (GST-tagged), Human recombinant
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Alternative_names
Bromodomain PHD Finger Transcription Factor
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Description
An essential regulator of gene expression
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥80%
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Assay
SDS-PAGE
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Molecular Weight
40.1 kDa (2796-2907 aa + NT GST Tag)
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM β-mercaptoethanol and 5% glycerol.
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Background Information
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BPTF is the largest component of the NURF chromatin remodeling complex. It includes adjacent PHD and bromodomains which recognize trimethylation of H3K4 or acetylation of lysines in histone 4, respectively. BPTF is an essential regulator of gene expression in early mouse embryos, and its knock-out is embryonic lethal. Duplication of the BPTF gene has been suggested to increase proliferation of cultured cancer cells, and aberrant BPTF copy numbers were found in 42% of the “NCI-60” panel of 60 human cancer cell lines. This protein product contains the bromodomain of BPTF.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MGST3, BPTF
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Short name
BPTF bromodomain (2796-2907 aa) (GST-tagged), recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
BPTF bromodomain (2796-2907 aa) (GST-tagged), H. sapiens Rec.
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Alternative technique
rec
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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