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Synonyms
L-Ascorbate, oxygen oxidoreductase, Ascorbase
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Alternative_names
L-Ascorbate, oxygen oxidoreductase, Ascorbase
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Description
Converts ascorbic acid to dehydroascorbic acid
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Source
Cucurbita sp.
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Activity Specifications test method
1,000 to 3,000 units per mg protein
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Unit Definition
One unit will oxidize 1.0 µmole of L-ascorbate to dehydroascorbate per minute at pH 5.6 at 25 °C.
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Molecular Weight
The enzyme exists as a dimer with a molecular weight of approximately 140 kDa. The monomer molecular weight is approximately 65 to 70 kDa. It is a multicopper oxidase containing approximately 8 copper atoms per molecule.
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Storage Temp
4.0 °C
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Shipping
Gel Pack
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Shelf Life
6 months
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Appearance
Lyophilized
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Physical form description
Lyophilized powder containing buffers and sucrose as stabilizer.
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Reconstitution Instructions
The enzyme is reconstituted in 4 mM sodium phosphate buffer, pH 5.6, with 0.05% (w/v) BSA.
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Background Information
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70 kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.This enzyme is involved in a redox system involving ascorbic acid. The enzyme is highly specific for L-ascorbic acid and a few analogs. The pH optimum for enzymatic activity is in the range of pH 5.5 to 7.0. The activity at pH 4.5 and 8.3 is approximately 50% maximal activity.
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Amino acid sequence
552 amino acid
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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