AO, apoplastic ascorbate oxidase

• Catalog number
  AS06 184
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized in PBS pH 7.4
• Immunogen
  ascorbate oxidase purified from Cucurbita sp.
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Total IgG
• How to reconstitute
  For reconstitution add 100 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS09 383 | anti-ascorbate oxidase biotinylated antibody
• Recommended dilutions for use
  1:2 000 with standard ECL (WB)
• Molecular weight expected аpparent
  61 | 45 kDa (Cucurbita pepo)
• Verified reactivity
  Cucurbita sp., Nicotiana tabacum, Solanum tuberosum, Zea mays
• Possible reactivity
  dicots including Glycine max, Cucumis melo, monocots including Oryza sativa
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  antibody can be used as a marker of apoplast
• References
  Kielbowicz et al. (2008) The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J. Expo Bot. 59:2191-2203.
• Scientific context
  Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
• Notes
  IgG concentration is 4 µg/µl
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  apoplastic   ascorbate   oxidase  
• Short name
  AO, apoplastic ascorbate oxidase
• Alternative name
  AO, apoplastic ascorbate oxidase

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