Anti-Human CD4 FITC Antibody (OKT4)
-
Catalog number
A1582-25
-
Price
134.66 USD
-
Size
25 tests
-
-
Antibody Target
CD4
-
Target Alternative Name
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD_antigen: CD4
-
Antibody Type
Monoclonal
-
Clone
OKT4
-
Species Reactivities
Human
-
Accession
P01730
-
Gene Id
920
-
Appearance
Colorless liquid
-
Form
Liquid
-
Formulation
Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.
-
Purification
Affinity chromatography.
-
Application
Flow cytometry
-
Positive Control
FACS: Human PMBCs
-
Application And Usages
Flow (Cell Surface): 5 µ (0.25 ug) l/1x106 cells per test.
-
Description
The OKT4 monoclonal antibody specifically binds to the CD4 receptor for the human immunodeficiency virus (HIV). CD4 is a 59 kDa single-chain transmembrane glycoprotein that expressed on the surface of most of the thymocytes, T-helper cells, and in low levels on monocytes and macrophages. CD4 is a co-receptor in the antigen-induced T cell activation (together with the MHC class II). The OKT4 and the RPA-T4 monoclonal antibodies recognize different epitopes of CD4 and they do not exhibit cross-block binding.
-
Storage Condition
4°C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans.
-
Handling
The antibody solution should be gently mixed before use.
-
Western Blot Verified
Not Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
FACS Verified
Not Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Dot Blot Verified
Not Verified
-
Flow Cytometry Verified
Verified
-
-
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Biovision supplies antibodies that are for research of human proteins.
-
Conjugation
Anti-FITC Antibody
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD4
-
Short name
Anti- CD4 FITC Antibody (OKT4)
-
Technique
anti-Human, Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Fluorescein
-
Isotype
Mouse IgG2b, kappa
-
Label
FITC
-
Species
Human, Humans
-
Alternative name
Antibody toHuman CD4 molecule fluorecein (antibody to-) (OKT4)
-
Alternative technique
antibodies, fluorescine
-
Alternative to gene target
CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
-
Gene info
-
Identity
-
Gene
-
Long gene name
CD4 molecule
-
Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
-
GenBank acession
-
Locus
-
Discovery year
1986-01-01
-
Entrez gene record
-
RefSeq identity
-
Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
-
VEGA ID
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products