Anti-HA Tag (FITC) Antibody

• Catalog number
  A1281-50
• Price
  Please ask
• Size
  50 µg

• Antibody Target
  HA
• Target Alternative Name
  Hemagglutinin, HA
• Antibody Type
  Monoclonal
• Clone
  5E11D8
• Immunogen Sequence
  A synthetic peptide YPYDVPDYA coupled to KLH
• Accession
  D5XIF4
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS, pH 7.4, 1% BSA and 0.02% sodium azide
• Purification
  Affinity purified
• Application
  IF, FC
• Positive Control
  HEK-293 cells expressing HA tagged protein
• Application And Usages
  IF: 0.5-2 µg/ml FC: 0.5-2 µg for 1 x 106 cells
• Description
  Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  Tag  
• Short name
  Anti-HA Tag (FITC) Antibody
• Technique
  Antibody, anti-, anti, FITC, antibody to, antibodies, antibodies against human proteins, antibodies for, Fluorescein, Tags
• Isotype
  MouseIgG1 ĸ
• Label
  tag
• Alternative name
  Antibody toHA detection labelled (fluorecein) (antibody to-)
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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